INDEX OF PROTOCOLS

    1. Growth of PC12 cells
    2. poly-L-lysine coating of plates for PC12 secretion and transfections
    3. Harvesting PC12 cells for reporter assay (DTOC)
    4. Harvesting PC12 cells for reporter assay (LT)
    5. Harvesting PC12 cells for reporter assay (LT_amended)
    6. Luciferase assay protocol
    7. [3H]-catecholamine secretion study
    8. Transfection of PC12 cells (Lipofectamine)
    9. Transfection of PC12 cells (Superfect)
    10. Transfection of PC12 cells (Lipofectin)
    11. Transfection of PC12 cells (GenePorter)
    12. Analysis of regulated secretion in PC12 cells (compilation of protocols 1-11)
    13. Metabolic labeling of cell proteins with [35S]-met
    14. Immunoprecipitation of metabolically labeled cells
    15. Immunoprecipitation for MALDI mass spectrometry
    16. 45Ca2+ cellular uptake
    17. CaPO4 Transfection for Chromaffin Cells
    18. Making antibodies to synthetic peptides
    19. Adrenal chromaffin granule (chromaffin vesicle) preparations
    20. Bovine chromaffin cell isolation
    21. Bovine chromaffin cell isolation (new version)
    22. 22Na+ uptake study in PC12 cells
    23. 45Ca2+ uptake study in PC12 cells
    24. N-acetyl-beta-D-glucosaminidase activity (lysosomal enzyme marker)
    25. Human or bovine chromogranin A (hCgA or bCgA) RIA
    26. Catecholamine assay (Weil-Malherbe fluorometric assay method)
    27. Succinate dehydrogenase ("succinate INT") enzymatic activity (mitochondrial enzyme marker)
    28. Dopamine beta-hydroxylase (DBH) enzymatic activity assay
    29. Equilibrium radioligand binding of [125I]-catestatin to Torpedo nicotinic receptor-containing membranes.
    30. Secretoneurin (SN) radioligand binding assay.
    31. Simplified fluorometric assay of catecholamine.
    32. Urine Kallikrein Assay
    33. Nitrate/Nitrite Assay for urine, serum or saliva
    34. Buccal cell DNA isolation
    35. Genomic DNA isolation
    36. Amendment to Genomic DNA isolation protocol
    37. Genomic DNA"mouthwash" protocol
    38. Pharmacogenomics and Hypertension Draft
    39. Buffy coat DNA prep
    40. Preparation of a paraformaldehyde fixative solution
    41. Chemiluminescence detection of chimeric embryonic alkaline phosphatase (EAP) secretion from PC12 cells
    42. Tips on resequencing human genomic DNA for polymorphism discovery
    43. Cheap urine creatinine assay

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